Assessment of cytogenetic indices in persons exposed to ionizing radiation
https://doi.org/10.25016/2541-7487-2014-0-1-70-76
Abstract
Cytogenetic analysis was performed in peripheral blood lymphocytes in different groups of persons who were exposed to ionizing radiation during their professional activities (61 persons), as well as in inhabitants of Semipalatinsk nuclear testing region (26 persons). Period of time from irradiation till cytogenetic analysis was from 1 to 49 years. Analysis of unstable chromosome aberrations revealed statistically increased frequency of all types of chromosome disturbances (3.25 ± 0.24) % in the group of exposed persons, including paired fragments (0.74 ± 0.11) % and cytogenetic radiation markers (0.36 ± 0.06) %, compared with control group (1.91 ± 0.20), (0.29 ± 0.05), (0.04 ± 0.02) %, respectively. Therefore, cytogenetic analysis performed many years after irradiation (up to 49 years) makes it possible to confirm the radiation exposure including emergencies.
About the Authors
E. G. Neronova
The Nikiforov Russian Center of Emergrency and Radiation Medicine, EMERCOM of Russia
Russian Federation
PhD on Biol. Sci., Head of Laboratory
Russian Federation
PhD on Biol. Sci., Head of Laboratory
S. S. Aleksanin
The Nikiforov Russian Center of Emergrency and Radiation Medicine, EMERCOM of Russia
Russian Federation
Dr. Med. Sci., Prof., Director
Russian Federation
Dr. Med. Sci., Prof., Director
References
1. Aleksanin S.S., Slozina N.M., Neronova E.G. Chrezvychainye situatsii i genom cheloveka [Emergency and the human genome]. Sankt_Peterburg. 2010. 84 p. (In Russ.)
2. Apsalikov K.N., Muldagaliev T.Zh., Belikhina T.I. [et al.]. Analiz i retrospektivnaya otsenka rezul’tatov tsitogeneticheskikh obsledovanii naseleniya Kazakhstana, podvergavshegosya radiatsionnomu vozdeistviyu v rezul’tate ispytanii yadernogo oruzhiya na Semipalatinskom poligone, i ikh potomkov [Analysis and retrospective evaluation of the results of cytogenetic examinations of Kazakhstan’s population exposed to radiation as a result of nuclear weapons testing at the Semipalatinsk test site, and their descendants]. Mediko4biologicheskie problemy zhiznedeyatel’nosti [Medical and biological problems of life activity]. 2013. N. 1. P. 42–49. (In Russ.)
3. Bochkov N.P., Chebotarev N.N. Nasledstvennost’ cheloveka i mutageny vneshney sredy [Human heredity and mutagens in the external environment]. Moskva. 1989. 270 p. (In Russ.)
4. Gigienicheskie kriterii sostoyaniya okruzhayushchei sredy. Rukovodstvo po izucheniyu geneticheskikh effektov v populyatsiyakh cheloveka [Hygienic criteria of environmental. Guide for the study of genetic effects in human populations]. Sovmestnoe izdanie Programmy OON po okruzhayushchei srede, Mezhdunarodnoi organizatsii truda i VOZ [A joint publication of the United Nations environment Programme, the International labour organization and the WHO]. Moskva. 1989. 122 p. (In Russ.)
5. Dubinina L.G. Leikotsity krovi cheloveka – test_sistema dlya otsenki mutagenov sredy [Human blood leukocytes –test_system for evaluation of environmental mutagens]. Moskva. 1977. 152 p. (In Russ.)
6. Khvostunov I.K., Shepel’ N.N., Sevan’kaev A.V. [et al.]. Sovershenstvovanie metodov biologicheskoi dozimetrii putem analiza khromosomnykh aberratsii v limfotsitakh krovi cheloveka pri obluchenii in vitro i in vivo [Perfection of methods of biological dosimetry by analysis of chromosomal aberrations in human blood lymphocytes under irradiation in vitro and in vivo]. Mediko4biologicheskie problemy zhiznedeyatel’nosti [Medical and biological problems of life activity]. 2013. N. 1. P. 135–147. (In Russ.)
7. Ponomareva A.V., Matveeva V.G., Osipova L.P., Posukh O.L. Khromosomnye narusheniya u zhitelei samburgskoi tundry v usloviyakh ekologicheskogo neblagopoluchiya [Chromosomal disorders among residents Samburg tundra in conditions of ecological trouble]. Sibirskiy Ekologicheskiy Zhurnal [Contemporary Problems of Ecology]. 2000. N 1. P. 67– 71. (In Russ.)
8. Analysis of FISH_painted chromosomes in individuals living near the Semipalatinsk nuclear test site / G. Stephan, S. Pressl, G. Koshpessova, B. I. Gusev // Radiat. Res. – 2001. – Vol. 155. – P. 796–800.
9. Anjaria K.B., Rao B.S. Chromosomal aberration analysis in chronically exposed radiation workers //J. Environ. Pathol. Toxicol. Oncol. – 2004. – Vol. 23. – P. 207–213.
10. Around Semipalatinsk nuclear test site: progress of dose estimations relevant to the consequences of nuclear tests / V.F. Stepanenko, M. Hoshi, I.K. Bailiff [et al.] / A summary of 3rd Dosimetry Workshop on the Semipalatinsk nuclear test site area, RIRBM, Hiroshima University, Hiroshima, 9–11 of March, 2005) // J. Radiat. Res. (Tokyo). – 2006. – Vol. 47, Suppl. A. – P. 1–13.
11. Biodosimetry study in Dolon and Chekoman villages in the vicinity of Semipalatinsk nuclear test site / N. Chaizhunusova, T.C. Yang, C. Land [et al.] // J. Radiat. Res. (Tokyo). – 2006. – Vol. 47, Suppl A. – P. A165–169.
12. Catoviс′ A., Tanackoviс′ F. Biological dosimetry – cytogenetics findings at persons occupationally exposed to ionizing radiation // Bosn. J. Basic. Med. Sci. – 2006. – Vol. 6, N 2. – P. 63–66.
13. Cytogenetic biomonitoring carried out in a village (Dolon) adjacent to the Semipalatinsk nuclear weapon test site / A. Testa, L. Stronati [et al.] // Radiat. Environ. Biophys. – 2001. – Vol. 40. – P. 125–129.
14. Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies. – Vienna, 2011. – 247 p.
15. Edwards A.A. The use of chromosomal aberrations in human lymphocytes for biological dosimetry // Radiat. Res. – 1997. – Vol. 148, N 5, Suppl. – P. 39–44.
16. Laliс′ H., Radoseviс′_Stasiс′ B. Chromosome aberrations in peripheral blood lymphocytes in subjects occupationally exposed to ionizing radiation or chemical clastogens // Folia Biol. (Praha). – 2002. – Vol. 48. – P. 102–107.
17. Neronova E., Slozina N., Nikiforov A. Chromosome alterations in cleanup workers sampled years after the Chernobyl accident // Radiat. Res. – 2003. – Vol. 160, N 1. – P. 46–51.
18. Simon S.L., Baverstock K.F., Lindholm C. A summary of evidence on radiation exposures received near to the Semipalatinsk nuclear weapons test site in Kazakhstan // Health. Phys. – 2003. – Vol. 84. – P. 718– 725.
19. Spectrum of chromosomal aberrations in peripheral lymphocytes of hospital workers occupationally exposed to low doses of ionizing radiation / F. Maffei, S. Angelini, G.C. Forti e [et al.] // Mutat. Res. – 2004. – Vol. 22. – P. 91–99.
20. Stable chromosome aberrations in the lymphocytes of a population living in the vicinity of the Semipalatinsk nuclear test site / S. Salomaa, C. Lindholm, M. Tankimanova [et al.] // Radiat. Res. – 2002. – Vol. 158. – P. 591–596.
21. The results of the cytogenetic examination of the inhabitants of population centers adjacent to the Semipalatinsk nuclear testing grounds (at the period of the work of the interdepartamental commission in 1989)] / A.V. Sevan'kaev, M.A. Ankina, E.V. Golub [et al.] // Radiats. Biol. Radioecol. – 1995. – Vol. 35. – P. 596–607.
22. Unstable_type chromosome aberrations in lymphocytes from individuals living near Semipalatinsk nuclear test site / K. Tanaka, S. Iida, N. Takeichi [et al.] // J. Radiat. Res. (Tokyo). – 2006. – Vol. 47, Suppl A. – P. 159–164.
23. Zakeri F., Assaei R.G. Cytogenetic monitoring of personnel working in angiocardiography laboratories in Iran hospitals. Cytogenetic monitoring of personnel working in angiocardiography laboratories in Iran hospitals // Mutat. Res. – 2004. – Vol. 562. – P. 1–9.
24. Zakeri F., Hirobe T.A. Cytogenetic approach to the effects of low levels of ionizing radiations on occupationally exposed individuals // Eur. J. Radiol. – 2010. – Vol. 73. – P. 191–195.
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For citations:
Neronova E.G., Aleksanin S.S. Assessment of cytogenetic indices in persons exposed to ionizing radiation. Medicо-Biological and Socio-Psychological Problems of Safety in Emergency Situations. 2014;(1):70-76. (In Russ.) https://doi.org/10.25016/2541-7487-2014-0-1-70-76
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