Neurophysiological analysis of brain cortex bioelectrical activity during acute hypoxia-hypercapnia in experiment
https://doi.org/10.25016/2541-7487-2014-0-1-64-69
Abstract
The somatosensory cortex single neurons activity was studied in cats when testing original hypoxia-hypercapnia model. 24 experiments were performed on cats with weight of 3.5-4.0 kg. Under aethaminalum- natrium narcosis (30-35 mg/kg introperitoneally), soft tissues of head were cut and trepanation holes were made in the skull. Then animals were intubated and unmoved by muscle relaxant for starting of artificial breathing. Evoked potentials and cortex neurons reactions were registered in response to electrical stimulation of contralateral radial nerve by single square-wave pulses with 5-7 V amplitude and 0.2 ms duration. Stage I hypoxia occurred in animals (7.1 ± 1.2) min after start of required model conditions of experiment, II - in (34.6 ± 3.3) min, III - in (50.3 ± 3.5) min, IV - usually in (55.9 ± 3.8) min. In studying of acute hypoxia-hypercapnia action on cat neurons impulse activity, 77 neurons were registered. Clear changes in spontaneous and evoked neurons activities were associated with the rise of hypoxic status in the experimental animals. In mild hypoxia (15 min), the spontaneous impulse frequency of neuron increased from 6 (baseline) to 21 per second. After intensification of hypoxic condition (II and III stages), further quantitative changes in spontaneous and evoked neuron activity were observed. Just before transition of animal to stage IV (terminal), the neuron (50 min of observation) had stopped generating action potentials, becoming practically unresponsive. These phasic changes of neuron activity helped to reveal the role of hypercapnia factor in the gradual process of acute hypoxia-hypercapnia in animals
About the Authors
D. V. Sosin
Smolensk State Medical Academy
Russian Federation
PhD on Med. Sci., Assistant Prof., Department of Normal Physiology
Russian Federation
PhD on Med. Sci., Assistant Prof., Department of Normal Physiology
A. V. Evseyev
Smolensk State Medical Academy
Russian Federation
Dr. Med. Sci., Prof. of the Normal Physiology Department
Russian Federation
Dr. Med. Sci., Prof. of the Normal Physiology Department
V. A. Pravdivtsev
Smolensk State Medical Academy
Russian Federation
Dr. Med. Sci., Prof. and Head, Department of Normal Physiology
Russian Federation
Dr. Med. Sci., Prof. and Head, Department of Normal Physiology
P. D. Shabanov
The Kirov Military Medical Academy
Russian Federation
Dr. Med. Sci., Prof. and Head, Department of Pharmacology
Russian Federation
Dr. Med. Sci., Prof. and Head, Department of Pharmacology
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For citations:
Sosin D.V., Evseyev A.V., Pravdivtsev V.A., Shabanov P.D. Neurophysiological analysis of brain cortex bioelectrical activity during acute hypoxia-hypercapnia in experiment. Medicо-Biological and Socio-Psychological Problems of Safety in Emergency Situations. 2014;(1):64-69. (In Russ.) https://doi.org/10.25016/2541-7487-2014-0-1-64-69
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