Experimental Evaluation of the Efficacy of Sodium Selenite as a Remedy for Wounds

Relevance. Emergencies, both of natural and anthropogenic origin, are accompanied by a large number of injuries and wounds, the treatment of which is associated with great difficulties due to frequent infectious complications and lethargic repair of damaged tissues. Promising approach to this problem is the use of antioxidants to reduce the severity of inflammation and stimulate repair. Intention. To evaluate the efficacy of topical application of the antioxidant composition based on selenium for acceleration of reparative processes in woundу. Methods. Experimental studies were carried out on 45 male Wistar rats. Wounds in rats were modeled under ether anesthesia by up to 4 cm excision of a pre-depilated area of the skin of the back to the underlying fascia. Wounds were treated by daily dressings with 0.5 % sodium selenite vs dimethyl sulfoxide solvent as a comparator. The presence and nature of the inflammatory reaction, the condition of edges and bottom of wounds, time to cleaning out necrotic tissues and the appearance of granulation, the nature of granulation tissue, time to the onset of epithelialization of wounds and the general condition of animals, the cytogram of wound prints, and the activity of antioxidant protection enzymes were evaluated in rats. Results and Discussion. It has been established that local use of sodium selenite has a stimulating effect on reparative processes in the wound due to increased activity of antioxidant enzymes. Thus, after 6-day treatment. sodium selenite increased catalase activity by 18.1 %, glutathione peroxidase by 63.2 %, glutathione reductase by 46.1 % compared to ointment with dimethyl sulfoxide. It was revealed that enhanced antioxidant protection of wound tissues accelerates maturation of granulations and wounds heal 1.6 times faster than in the control group. Conclusion. Medicinal compositions with sodium selenite are promising for local treatment of wounds.

1. Admakin A.L., Sokolov V.A., Sannikov V.M., Ushakov M.V. Aktual’nye voprosy reabilitatsii postradavshikh s ozhogami i ranami razlichnogo geneza [Topical issues of rehabilitation of the injured with burns and wounds of different genesis]. Mediko-biologicheskie i sotsial’no-psikhologicheskie problemy bezopasnosti v chrezvychainykh situatsiyakh [Medical-Biological and Socio-Psychological Problems of Safety in Emergency Situations]. 2011. N 4. Pp. 41–45. (In Russ.)

2. Belyaev A.N., Rygin A.N., Zakhvatov A.N. Sistemnaya i regional’naya antioksidantnaya terapiya pri oslozhnennykh for makh diabeticheskoi stopy [System and regional antioxidant therapy for complicated diabetic foot]. Khirurgiya. Zhurnal imeni N.I. Pirogova [Surgery. Journal named after N.I. Pirogov]. 2007. N 11. Pp. 46–50. (In Russ.)

3. Galkin A.A., Demidova V.S. Neitrofily i sindrom sistemnogo vospalitel’nogo otveta [Neutrophils and systemic inflammatory response syndrome]. Rany i ranevaya infektsiya. Zhurnal im. professora B.M. Kostyuchenok [Wounds and Wound Infec tions. B.M. Kostyuchyonok Journal]. 2015. N 2. Pp. 25–32. (In Russ.)

4. Glukhov A.A., Alekseeva N.T., Lobtsov A.V. Kliniko-morfologicheskoe obosnovanie primeneniya gidropressivnoi sanatsii i polyarizovannogo oblucheniya pri lechenii ran myagkikh tkanei v eksperimente [Clinical and morphological substantiation of hydropressive technologies and polarized light therapy in the treatment of soft tissue wounds in experiment]. Vestnik eksperimental’noi i klinicheskoi khirurgii [Bulletin of Experimental and Clinical Surgery]. 2010. Vol. 3, N 2. Pp. 133–145. (In Russ.)

5. Karpishchenko A.I., Andreev V.A., Antonov V.G. Meditsinskaya laboratornaya diagnostika [Medical laboratory diagnostics]. Moskva. 2014. 434 p. (In Russ.)

6. Kogaladze R.A. Rabota s laboratornymi zhivotnymi v kontekste bioetiki – istoriya, sovremennost’, perspektivy [The experiments with laboratory animals from bioethics point of view – history, current state, perspectives]. Uspekhi fiziologicheskikh nauk [Progress in Physiology]. 2004. Vol. 35, N 2. Pp. 92–109. (In Russ.)

7. Korolyuk M.A., Ivanova L.I., Maiorova I.G., Tokareva V.E. Metod opredeleniya aktivnosti katalazy [The method for determining the activity of catalase]. Laboratornoe delo = Klinicheskaya laboratornaya diagnostika [Clinical Laboratory Services]. 1988. N 1. Pp. 16–19. (In Russ.)

8. Kostyakov D.V., Zinovyev E.V. Sovremennye vozmozhnosti vybora patogeneticheski obosnovannykh metodik lecheniya ukushennykh ran [Modern choice of pathogenetically-based methods of treatment of bite wounds]. Vestnik Rossiiskoi voenno-meditsinskoi akademii [Bulletin of Russian Military Medical Academy]. 2016. N 2. Pp. 235–240. (In Russ.)

9. Legeza V.I., Grebenyuk A.N., Kondakov A.Yu., Zargarova N.I. Sravnitel’naya otsenka effektivnosti ranozazhivlyayushchikh sredstv pri mestnykh i sochetannykh radiatsionnykh porazheniyakh v eksperimente [Comparative evaluation of healing remedies for local and combined radiation injury in an experiment]. Radiatsionnaya biologiya. Radioekologiya [Radiation Biology. Radioecologу]. 2015. Vol. 55, N 6. Pp. 584–590. (In Russ.)

10. Loktionov P.V., Gudz’ Yu.V. Opyt lecheniya ran nizhnikh konechnostei s obshirnoi travmaticheskoi otsloikoi kozhi i podkozhnoi kletchatki [Experience of treating wounds of the lower extremities with extensive traumatic detachment of skin and subcutaneous tissue]. Mediko-biologicheskie i sotsial’no-psikhologicheskie problemy bezopasnosti v chrezvychainykh situatsiyakh [Medico-Biological and Socio-Psychological Problems of Safety in Emergency Situations]. 2015. N 1. Pp. 22–29. (In Russ.)

11. Patent N 2369395, RU, MPI A61K33/04. Sredstvo dlya stimulyatsii reparativnykh protsessov v rane [Remedy for stimulation of reparative processes in a wound]. Namokonov E.V., Miromanov A.M., Lutsenko V.N. [et al.]. N 2008103046/15, Declared 28.01.2008 ; Published 10.10.2009, Bulletin 10. (In Russ.)

12. Privol’nov V.V. Vybor preparata dlya mestnogo lecheniya infitsirovannykh ran [Choice of an agent for topical treatment of infected wounds]. Rany i ranevaya infektsiya. Zhurnal im. professora B.M. Kostyuchenok [Wounds and Wound Infections. B.M. Kostyuchyonok Journal]. 2015. N 1. Pp. 13–18. (In Russ.)

13. Tarumov R.A., Grebenyuk A.N., Basharin V.A., Kovtun V.Yu. Biologicheskie svoistva fitoestrogena genisteina (obzor literatury) [Biological properties of phytoestrogen genistein (review of publications)]. Meditsina ekstremal’nykh situatsii [Medicine of Extreme Situations]. 2014. N 2. Pp. 55–68. (In Russ.)

14. Shval’b P.G., Kalinin R.E. Antioksidantnaya zashchita i funktsional’noe sostoyanie endoteliya u bol’nykh obliteriruyushchim aterosklerozom arterii nizhnikh konechnostei do i posle operativnogo lecheniya [Antioxidant activity and functional endothelium characteristics before and after surgery in patients with atherosclerosis obliterans]. Khirurgiya. Zhurnal imeni N.I. Pirogova [Surgery. Journal named after N.I. Pirogov]. 2009. N 1. Pp. 53–55. (In Russ.)

15. Yartseva A.A., Antushevich A.E., Grebenyuk A.N. Eksperimental’noe izuchenie mekhanizmov gemostimuliruyushchei aktivnosti organicheskoi soli disul’fida glutationa i inozina v usloviyakh ostrogo radiatsionnogo vozdeistviya [Experimental study of glutathione disulfide organic salt and inosine hemostimulating activity mechanisms under acute radiation exposure]. Mediko-biologicheskie i sotsial’no-psikhologicheskie problemy bezopasnosti v chrezvychainykh situatsiyakh [Medico-Biological and Socio-Psychological Problems of Safety in Emergency Situations]. 2016. N 1. Pp. 79–85. (In Russ.)

16. Blueman D., Bluefield C. The use of larval therapy to reduce the bacterial load in chronic wounds. J. Wound Care. 2012. Vol. 21, N 5. Рp. 244–253.

17. Carroll L., Davies M.J., Pattison D.I. Reaction of low-molecular-mass organoselenium compounds (and their sulphur analogues) with inflammation-associated oxidants. Free Radical Res. 2015. Vol. 49, N 6. Pp. 750–767.

18. Dowsett C., Davis L., Henderson V., Searle R. The economic benefits of negative pressure wound therapy in community-based wound care in the NHS. Int. Wound J. 2012. Vol. 9, N 5. Pp. 544–552.

19. Gottrup F. Trends in surgical wound healing. Scand. J. Surg. 2008. Vol. 97. Pp. 220–225.

20. Sen Ch.K., Gordillo G.M., Roy S. [et al.]. Human skin wounds: a major and snowballing threat to public health and the economy. Wound Repair Regen. 2009. Vol. 17, N 6. Pp. 763–771.

21.