MICROENVIRONMENT IN HUMAN HEALTH AND DISEASE BY MASS SPECTROMETRY OF MICROBIAL MARKERS

The review of data on the use of mass spectrometry of microbial markers (MSMM)  to  study the  micro ecology of man  is provided. The method gives  a new version of the  molecular microbiological testing by allowing the  simultaneous quantification of more than a  hundred microbial markers directly in biological samples without  prior  cultivation of microorganisms and  the use of biochemical testing materials / genetic primers. Getting real  time advanced information about anaerobes and  aerobes which are hard to cultivate, as well as actinobacteria, viruses, yeasts and  fungi from one  sample provides a comprehensive understanding of the microbial etiology of the disease. We obtained a new confirmation of polymicrobial nature of infectious processes, as well as conventional division  into pathogenic and  nonpathogenic microbes. MSMM data suggest that  anaerobic genera Clostridium, Eubacterium, Propionibacterium, and  actinobacteria Streptomyces, Nocardia, Rhodococcus dominate in polymicrobial infections.  Translocation  of  these organisms with Lactobacillus and Bifidobacterium from the intestine into sites of inflammation has been proved. In inflamed organ, the  concentration of their  markers is greater than  in the blood of the same patient, which indicates their reproduction in the organ itself. Quanti tative  measurements by MSMM allow us  to study the dynamics of changes in the microbiota during therapeutic activities, including the  influence of antibiotics and probiotics on intestinal parietal microbiota.

mass spectrometry, microbial markers, microenvironment, microbiota, microorganisms, microecological status, infection

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