Neuromorphological correlates of low-dose radiation effects
https://doi.org/10.25016/2541-7487-2016-0-1-71-78
Abstract
White outbred male rats (270 animals) at the age of 4 months, were irradiated with gamma-rays 60Co at doses of 10; 20; 50 and 100 cGy with a dose rate of 0,5 Gy/h. Fragments of the brain were taken on day 1 and at months 6, 12, and 24 of post-radiation period. Following standard histological processing, tinctorial changes of neurons were assessed along with their morphometric parameters, total protein content and nucleic acids. It was found that by the end of the follow-up period the number of normochromic neurons reduced and the number of hyper- and hypochromic cells and their destructive forms increased. Nerve Cell index decreased over time, indicating the death of the neurons, but without statistically significant reduction in their number in the area. In the post-radiation period, phase changes in size of the cytoplasm, nucleus and nucleolus as well as content of nucleic acids (RNA in the cytoplasm and nucleoli, DNA in the nuclei) were observed. In irradiated animals, these were more pronounced and not always the unidirectional with age-related changes nature. This creates a certain instability in the structural and functional organization of neurons, which, with predisposing factors, may induce functional disorders of the central nervous system.
About the Authors
I. B. Ushakov
Nikiforov Russian Center of Emergency and Radiation Medicine, EMERCOM of Russia
Russian Federation
Russian Federation
Dr. Med. Sci, Prof., Member of Russian Academy of Sciences, the Nikiforov Russian Center of Emergency and Radiation Medicine, EMERCOM of Russia (Russia, 194044, Saint-Petersburg, Academica Lebedeva Str., 4/2)
V. P. Fedorov
Voronezh State Medical University named after N. N. Burdenko
Russian Federation
Russian Federation
Dr. Med. Sci. Prof., Department of Normal Anatomy, Voronezh State Medical University named after N. N. Burdenko (Russia, 394036, Voronezh, Studencheskaya Str., 10)
O. P. Gundarova
Voronezh State Medical University named after N. N. Burdenko
Russian Federation
Russian Federation
teacher, Department of Normal Anatomy, Voronezh State Medical University named after N. N. Burdenko (Russia, 394036, Voronezh, Studencheskaya Str., 10)
N. V. Sgibneva
Voronezh State Medical University named after N. N. Burdenko
Russian Federation
Russian Federation
PhD Biol. Sci., teacher, Department of Normal Anatomy, Voronezh State Medical University named after N. N. Burdenko (Russia, 394036, Voronezh, Studencheskaya Str., 10)
N. V. Maslov
Voronezh State Medical University named after N. N. Burdenko
Russian Federation
Russian Federation
PhD Med. Sci., teacher, Department of Normal Anatomy, Voronezh State Medical University named after N. N. Burdenko (Russia, 394036, Voronezh, Studencheskaya Str., 10)
N. V. Fedorov
Voronezh State Medical University named after N. N. Burdenko
Russian Federation
Russian Federation
PhD Med. Sci., teacher, Department of Normal Anatomy, Voronezh State Medical University named after N. N. Burdenko (Russia, 394036, Voronezh, Studencheskaya Str., 10)
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Review
For citations:
Ushakov I.B., Fedorov V.P., Gundarova O.P., Sgibneva N.V., Maslov N.V., Fedorov N.V. Neuromorphological correlates of low-dose radiation effects. Medicо-Biological and Socio-Psychological Problems of Safety in Emergency Situations. 2016;(1):71-78. (In Russ.) https://doi.org/10.25016/2541-7487-2016-0-1-71-78
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