References

mchsros

Медико-биологические и социально-психологические проблемы безопасности в чрезвычайных ситуациях

Medicо-Biological and Socio-Psychological Problems of Safety in Emergency Situations

1995-44412541-7487

«The Nikiforov Russian Center of Emergency and Radiation Medicine»

10.25016/2541-7487-2022-0-2-82-92

mchsros-807

Research Article

Медицинские проблемы

Medical Issues

Возможности применения биоинженерных заменителей кожи в комбустиологии (обзор литературы)

The possibilities of using bioengineered skin substitutes in combustiology (literature review)

https://orcid.org/0000-0002-5630-5247

Шаповалов

С. Г.

Shapovalov

S. G.

Шаповалов Сергей Георгиевич – д-р мед. наук доц., зав. отд-нием ожоговой травмы и пластич. хирургии

194044, Санкт-Петербург, ул. Акад. Лебедева, д.4/2

Sergey Georgievich Shapovalov – Dr. Med. Sci. Associate Prof., Head of the Department of Burn Trauma and Plastic Surgery

4/2, Academiсa Lebedeva Str., St. Petersburg, 194044

shapovalov_serg@mail.ru

https://orcid.org/0000-0002-5889-3564

Кчеусо

А. В.

Kcheuso

A. V.

Кчеусо Александр Викторович – хирург-онколог

194044, Санкт-Петербург, ул. Акад. Лебедева, д.4/2

Aleksandr Victorovich Kcheuso – surgeon-oncologist

4/2, Academiсa Lebedeva Str., St. Petersburg, 194044

kcheuso@mail.ru

https://orcid.org/0000-0002-6399-6963

Кошелев

Т. Е.

Koshelev

T. E.

Кошелев Тарас Евгеньевич – канд. мед. наук доц., каф. общ. хирургии

191015, Санкт-Петербург, ул. Кирочная, д.41

Taras Evgenyevich Koshelev – PhD Med. Sci., Associate Prof. of the Department of General surgery

41, Kirochnaya Str., St. Petersburg, 191015

kte@yandex.ru

https://orcid.org/0000-0003-4626-1639

Савченков

Д. К.

Savchenkov

D. K.

Савченков Дмитрий Константинович – канд. мед. наук, ассистент каф. общ. хирургии

191015, Санкт-Петербург, ул. Кирочная, д.41

Dmitry Konstantinovich Savchenkov – PhD Med. Sci., lecturer, the Department of General surgery

41, Kirochnaya Str., St. Petersburg, 191015

savchenkov.dmitrij@gmail.com

Всероссийский центр экстренной и радиационной медицины им. А.М.Никифорова МЧС РоссииРоссияThe Nikiforov Russian Center of Emergency and Radiation MedicineRussian Federation

Северо-Западный государственный медицинский университет им. И.И. МечниковаРоссияNorth-Western State Medical University named after I.I. MechnikovRussian Federation

2022

03082022

028292

2022

Шаповалов С.Г., Кчеусо А.В., Кошелев Т.Е., Савченков Д.К.

Shapovalov S.G., Kcheuso A.V., Koshelev T.E., Savchenkov D.K.

Данная работа распространяется под лицензией Creative Commons Attribution 4.0.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://mchsros.elpub.ru/jour/article/view/807

Актуальность. Несмотря на научно-технический прогресс и совершенствование методов лечения, оказание помощи при термических ожогах кожного покрова остается сложной многокомпонентной проблемой. Обширные глубокие ожоги не способны к самостоятельному заживлению, в связи с чем за последнее столетие стандартным методом лечения стала аутодермопластика. Однако дефицит здоровых тканей пациента часто не позволяет выполнить трансплантацию в полном объеме, что влечет за собой необходимость поиска вариантов замены аутотканей.Цель – представить современные возможности и оценить проблемы применения биоинженерных заменителей кожи в комбустиологии.Методология. Проведен поиск научных литературных источников, опубликованных в последние годы и представленных в базе PubMed и на платформе Научной электронной библиотеки (eLIBRARY.ru). Результаты и их анализ. В настоящее время разработано множество заменителей кожи, начиная от однослойных тканевых эквивалентов до искусственной кожи и генетически модифицированных заменителей. Однако все они еще далеки от совершенства, имеют свои достоинства и недостатки, которые определяют особенности их применения и приводят к необходимости продолжения научного поиска.Заключение. Разработка тканевых эквивалентов кожи позволила значительно улучшить результаты лечения пострадавших с глубокими ожогами и является перспективным направлением в развитии современной комбустиологии.

Relevance. Despite scientific and technological progress and improvement of treatment methods, providing assistance for thermal burns of the skin remains a complex multicomponent problem. Extensive deep burns are not capable of self-healing, and therefore, over the past century, autodermoplasty has become the standard method of treatment. However, the shortage of healthy tissues of the patient often does not allow the transplantation to be performed in full, which entails the need to search for options for replacing auto-tissues.Intention. To present modern possibilities and evaluate the problems of using bioengineered skin substitutes in kombustiology.Methodology. Scientific literary sources published in recent years were searched for using the PubMed database and the platform of Scientific Electronic Library (eLIBRARY.ru ).Results and Discussion. Currently, many skin substitutes have been developed, ranging from single-layer tissue equivalents to artificial leather and genetically modified substitutes. However, all of them are still far from perfect, they have their advantages and disadvantages, which determine the features of their application and necessitate further research.Conclusion. The development of tissue equivalents of the skin has significantly improved the results of treatment of victims with deep burns and is a promising direction in the development of modern combustiology.

ожогзаменители кожитканевая инженериявнеклеточный матрикскератиноцитыфибробласты

burnsskin substitutestissue engineeringextracellular matrixkeratinocytesfibroblasts

References1

Богданов С.Б., Каракулев А.В., Поляков А.В. [и др.]. Совершенствование комплексного применения клеточной терапии и биологических раневых покрытий в лечении пациентов с дефектами кожных покровов // Пластич. хирургия и эстетич. медицина. 2019. № 4. С. 43–49.

Bogdanov S.B., Karakulev A.V., Poljakov A.V. [et al.]. Sovershenstvovanie kompleksnogo primenenija kletochnoj terapii i biologicheskih ranevyh pokrytij v lechenii pacientov s defektami kozhnyh pokrovov [Cell therapy and biological wound coatings in the treatment of patients with skin defects]. Plasticheskaja hirurgija i jesteticheskaja medicina [Journal of Plastic Surgery and Aesthetic Medicine]. 2019; (4):43–49. (In Russ.)

Гаврилюк Б.К., Рочев Ю.А., Николаева Т.И. Культура клеток и реконструкция ткани: (на примере кожи). Пущино : Изд-во ОНТИ НЦБИ АН СССР, 1988. 124 с.

Gavriljuk B.K., Rochev Ju.A., Nikolaeva T.I. Kul’tura kletok i rekonstrukcija tkani: (Na primere kozhi) [Cell culture and tissue reconstruction: (Using the example of skin)]. Pushchino. 1988. 124 p. (In Russ.)

Гилевич И.В. , Федоренко Т.В., Коломийцева Е.А. [и др.]. Достижения клеточной терапии в комбустиологии // Инновац. медицина Кубани. 2017. Т. 6, № 2. С. 6–7.

Gilevich I.V., Fedorenko T.V., Kolomiitseva E.A. [et al.]. Dostizheniya kletochnoi terapii v kombustiologii [Cell therapy advances in combustiology]. Innovatsionnaya meditsina Kubani [Innovative medicine of Kuban]. 2017; 6(2):6–7. (In Russ.)

Потекаев Н.Н. Фриго Н.В., Петерсен Е.В. Искусственная кожа: виды, области применения // Клинич. дерматология и венерология. 2017. № 6. С. 7–15.

Potekaev N.N. Frigo N.V., Petersen E.V. Iskusstvennaya kozha: vidy, oblasti primeneniya [Artificial skin: types and applications]. Klinicheskaya dermatologiya i venerologiya [Russian journal of clinical dermatology and venereology]. 2017; (2):7–15. (In Russ.)

Расулов М.Ф., Васильченков А.В., Крашенинников М.Е. [и др.]. Трансплантация стволовых клеток костного мозга в лечении ожоговых ран // Тихоокеан. мед. журн. 2004. № 4. С. 32–33.

Rasulov M.F., Vasil’chenkov A.V., Krasheninnikov M.E. [et al.]. Transplantatsiya stvolovykh kletok kostnogo mozga v lechenii ozhogovykh ran [Bone marrow stem cell transplantation in the therapy of burn wounds]. Tikhookeanskii meditsinskii zhurnal [Pacific medical journal]. 2004; (4):32–33. (In Russ.)

Саркисов Д.С., Алексеев А.А., Глущенко Е.В. Теоретические и практические аспекты использования культивированных фибробластов при восстановлении целостности кожного покрова // Вестн. Рос. акад. мед. наук. 1994. № 7. С. 6–11.

Sarkisov D.S., Alekseev A.A., Glushchenko E.V. Teoreticheskie i prakticheskie aspekty ispol’zovaniya kul’tivirovannykh fibroblastov pri vosstanovlenii tselostnosti kozhnogo pokrova [Theoretical and practical aspects of the use of cultured fibroblasts in skin integrity regeneration]. Vestnik Rossiiskoi akademii meditsinskikh nauk [Annals of the Russian academy of medical sciences]. 1994; (7):6–11. (In Russ.)

Сачков А.В., Боровкова Н.В., Жиркова Е.А. [и др.]. Использование трупной кожи в лечении ран // Трансплантология. 2018. T. 4, № 10. С. 327–335. DOI: 10.23873/2074-0506-2018-10-4-327-335

Sachkov A.V., Borovkova N.V., Zhirkova E.A. [et al.]. Ispol’zovanie trupnoi kozhi v lechenii ran [Use of cadaver skin in the treatment of wounds]. Transplantologiya [Transplantologiya. The Russian journal of transplantation]. 2018; 4(10):327–335. DOI: 10.23873/2074-0506-2018-10-4-327-335 (In Russ.)

Abdel-Sayed P., Hirt-Burri N., de Buys Roessingh A., Raffoul W., Applegate L.A. Evolution of Biological Bandages as First Cover for Burn Patients. Adv Wound Care (New Rochelle). 2019; 8(11):555–564. DOI: 10.1089/wound.2019.1037.

Alpeeva, E., Sukhanov, Y., Vorotelyak, E. Almost 40 Years of Tissue Engineering in Russia: Where Are We Now? Biomedicines. 2020; 8(2):25.

Austin R.E., Merchant N., Shahrokhi S., Jeschke M.G. A comparison of Biobrane and cadaveric allograft for temporizing the acute burn wound: Cost and procedural time. Burns. 2015; 41(4):749–753. DOI: 10.1016/j.burns.2014.10.003.

Bairagi A., Griffin B., Tyack Z. [et al.]. Comparative effectiveness of Biobrane®, RECELL® Autologous skin Cell suspension and Silver dressings in partial thickness paediatric burns: BRACS randomised trial protocol. Burns Trauma. 2019; 31(7):33. DOI: 10.1186/s41038-019-0165-0.

Balasubramani M., Kumar T.R., Babu M. Skin substitutes: a review. Burns. 2001; 27(5):534–544. DOI: 10.1016/s0305-4179(01)00018-3.

Bloemen M.C., van der Wal M.B., Verhaegen P.D. [et al.]. Clinical effectiveness of dermal substitution in burns by topical negative pressure: a multicenter randomized controlled trial. Wound Repair Regen. 2012; 20(6):797–805. DOI: 10.1111/j.1524-475X.2012.00845.x.

Burke J.F., Yannas I.V., Quinby WC Jr. [et al.]. Successful use of a physiologically acceptable artificial skin in the treatment of extensive burn injury. Ann Surg. 1981; 194(4):413–428. DOI: 10.1097/00000658-198110000-00005.

Chua A.W., Khoo Y.C., Tan B.K. [et al.]. Skin tissue engineering advances in severe burns: review and therapeutic applications. Burns Trauma. 2016; 4:3. DOI: 10.1186/s41038-016-0027-y.eCollection 2016.

Davis J.S. Address of the president: the story of plastic surgery. Ann Surg. 1941; 113(5): 641–656. DOI: 10.1097/00000658-194105000-00001.

Debels H., Hamdi M., Abberton K., Morrison W. Dermal matrices and bioengineered skin substitutes: a critical review of current options. Plast Reconstr Surg Glob Open. 2015; 3(1):284. DOI: 10.1097/GOX.0000000000000219.

Domaszewska-Szostek A.P., Krzyżanowska M.O., Czarnecka A.M., Siemionow M. Local Treatment of Burns with Cell-Based Therapies Tested in Clinical Studies. Journal of clinical medicine. 2021; 10(3):396.

Fan C., Pek C.H., Por Y.C., Lim G.J.S. Biobrane dressing for paediatric burns in singapore: A retrospective review. Singap. Med. J. 2018; (59):360–365. DOI: 10.11622/smedj.2017116.

Gardien K.L., Marck R.E., Bloemen M.C. [et al.]. Dutch Outback Study Group1. Outcome of Burns Treated With Autologous Cultured Proliferating Epidermal Cells: A Prospective Randomized Multicenter Intrapatient Comparative Trial. Cell Transplant. 2016; 25(3):437–448. DOI: 10.3727/096368915X689569.

Gore M.A., Akolekar D. Evaluation of banana leaf dressing for partial thickness burn wounds. Burns. 2003; 29(5):487–492. DOI: 10.1016/s0305-4179(03)00050-0.

O’Connor N.E., Mulliken J.B., Banks-Schlegel S., Kehinde O., Green H. Grafting of burns with cultured epithelium prepared from autologous epidermal cells. Lancet. 1981; 1(8211):75–78.

Gravante G., Di Fede M.C., Araco A. [et al.]. A randomized trial comparing ReCell system of epidermal cells delivery versus classic skin grafts for the treatment of deep partial thickness burns. Burns. 2007; 33(8):966–972. DOI: 10.1016/j.burns.2007.04.011.

Greenwood J.E., Clausen J., Kavanagh S. Experience with biobrane: uses and caveats for success. Eplasty. 2009; 9:(e25).

Halim A.S., Khoo T.L., Mohd Yussof S.J. Biologic and synthetic skin substitutes: An overview. Indian J. Plast Surg. 2010; 43 (Suppl): 23–28. DOI: 10.4103/0970-0358.70712.

Hu Z., Yang P., Zhou C. [et al.]. Marine Collagen Peptides from the Skin of Nile Tilapia (Oreochromis niloticus): Characterization and Wound Healing Evaluation. Mar Drugs. 2017; 15(4):102. DOI: 10.3390/md15040102.

Kaddoura I., Abu-Sittah G., Ibrahim A. [et al.]. Burn injury: review of pathophysiology and therapeutic modalities in major burns. Annals of burns and fire disasters. 2017; 30(2): 95–102.

Kaur A., Midha S., Giri S., Mohanty S. Functional Skin Grafts: Where Biomaterials Meet Stem Cells. Stem cells international. 2019; 2019;1–21.

Kirsner R.S., Sabolinski M.L., Parsons N.B. [et al.]. Comparative effectiveness of a bioengineered living cellular construct vs. a dehydrated human amniotic membrane allograft for the treatment of diabetic foot ulcers in a real world setting. Wound Repair Regen. 2015; 23:737–744. DOI: 10.1111/wrr.12332.

Kumar P. Classification of skin substitutes. Burns. 2008; 34(1):148–149. DOI: 10.1016/j.burns.2007.04.016

Kumar R.J., Kimble R.M., Boots R., Pegg S.P. Treatment of partial thicness burns: A prospective, randomized trial using Transcyte. ANZ J. Surg. 2004; 74(8):622–626. DOI: 10.1111/j.1445-1433.2004.03106.x.

Lee H. Outcomes of sprayed cultured epithelial autografts for full-thickness wounds: a single-centre experience. Burns. 2012; 38(6):931–936. DOI: 10.1016/j.burns.2012.01.014.

Lagus H., Sarlomo-Rikala M., Böhling T., Vuola J. Prospective study on burns treated with Integra®, a cellulose sponge and split thickness skin graft: Comparative clinical and histological study–Randomized controlled trial. Burns. 2013; 39:1577–1587. DOI: 10.1016/j.burns.2013.04.023.

Mansilla E., Marín G.H., Berges M. [et al.]. Cadaveric bone marrow mesenchymal stem cells: first experience treating a patient with large severe burns. Burns Trauma. 2015; 18(3):17. DOI: 10.1186/s41038-015-0018-4.

Oualla-Bachiri W., Fernández-González A., Quiñones-Vico M.I., Arias-Santiago S. From Grafts to Human Bioengineered Vascularized Skin Substitutes. International journal of molecular sciences. 2020; 21(21):8197. DOI: 10.3390/ijms21218197.

Philandrianos C., Andrac-Meyer L., Mordon S. [et al.]. Comparison of five dermal substitutes in full-thickness skin wound healing in a porcine model. Burns. 2012; 38(6):820–829. DOI: 10.1016/j.burns.2012.02.008

Pourchet L.J., Thepot A., Albouy M. [et al.]. Human Skin 3D Bioprinting Using Scaffold-Free Approach. Adv Healthc Mater. 2017; 6(4):38. DOI: 10.1002/adhm.201601101

Rheinwald J.G., Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell. 1975; 6(3):331–343. DOI: 10.1016/s0092-8674(75)80001-8.

Ryssel H., Gazyakan E., Germann G., Ohlbauer M. The use of MatriDerm in early excision and simultaneous autologous skin grafting in burns – a pilot study. Burns. 2008; 34(1):93–97. DOI: 10.1016/j.burns.2007.01.018.

Savoji H., Godau B., Hassani M.S., Akbari M. Skin Tissue Substitutes and Biomaterial Risk Assessment and Testing. Frontiers in bioengineering and biotechnology. 2018; 6:86. DOI: 10.3389/fbioe.2018.00086.

Shahrokhi S., Arno A., Jeschke M.G. The use of dermal substitutes in burn surgery: acute phase. Wound Repair Regen. 2014; 22(1):14–22. DOI: 10.1111/wrr.12119.

Still J., Glat P., Silverstein P. [et al.]. The use of a collagen sponge/living cell composite material to treat donor sites in burn patients. Burns. 2003; 29(8):837–841. DOI: 10.1016/s0305-4179(03)00164-5.

Sullivan T.P., Eaglstein W.H., Davis S.C., Mertz P. Sullivan The pig as a model for human wound healing. Wound Repair Regen. 2001; 9(2);66–76. DOI: 10.1046/j.1524-475x.2001.00066.x.

Troy J., Karlnoski R., Downes K. [et al.]. The Use of EZ Derm® in Partial-Thickness Burns: An Institutional Review of 157 Patients. Eplasty. 2013: 13:e14.

Tumanov V.P., Pal’tsyn A.A., Sarkisov D.S. Plastic operations of burns using cultured epithelium. Acta Chir. Plast. 1989; 31(1):15–21.

Urciuolo F., Casale C., Imparato G., Netti P.A. Bioengineered Skin Substitutes: the Role of Extracellular Matrix and Vascularization in the Healing of Deep Wounds. Journal of clinical medicine. 2019; 8(12):2083. DOI: 10.3390/jcm8122083.

Widjaja W., Tan J., Maitz P.K.M. Efficacy of dermal substitute on deep dermal to full thickness burn injury: a systematic review. ANZ J Surg. 2017; 87(6):446–452. DOI: 10.1111/ans.13920.

Wood F., Martin L., Lewis D. [et al.]. A prospective randomised clinical pilot study to compare the effectiveness of Biobrane® synthetic wound dressing, with or without autologous cell suspension, to the local standard treatment regimen in paediatric scald injuries. Burns. 2012; 38(6):830–839. DOI: 10.1016/j.burns.2011.12.020.

Yannas I.V., Burke J.F., Huang C., Gordon P.L. Correlation of in vivo collagen degradation rate with in vitro measurements. J. Biomed Mater Res. 1975; 9(6):623–628. DOI: 10.1002/jbm.820090608

The authors declare that there are no conflicts of interest present.